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F5875

Sigma-Aldrich

Ferredoxin solution from Spinacia oleracea (spinach)

buffered aqueous solution

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About This Item

Numéro CAS:
9040-09-9
Numéro MDL:
MFCD00131049
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.61

Source biologique

spinach (Spinacia oleracea)

Niveau de qualité

200

Forme

buffered aqueous solution

Concentration

1.0-3.0 mg/mL in 0.15 M Trizma buffer, pH 7.5 and NaCl (Frozen)

Application(s)

cell analysis

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

spinach ... SOVF(137530)

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Application

Ferredoxin has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium. Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation.

Actions biochimiques/physiologiques

Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.

Conditionnement

Package sizes are based on ferredoxin content.

Notes préparatoires

Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Rosa M Martínez-Espinosa et al.
Biochimica et biophysica acta, 1623(1), 47-51 (2003-09-06)
Haloferax mediterranei is a halophilic archaeon that can grow in aerobic conditions with nitrate as sole nitrogen source. The electron donor in the aerobic nitrate reduction to ammonium was a ferredoxin. This ferredoxin has been purified and characterised. Air-oxidized H.
Basilio Zafrilla et al.
Biochemical Society transactions, 39(6), 1844-1848 (2011-11-23)
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO₃⁻/NO₂⁻ reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in
Rosa María Martínez-Espinosa et al.
FEMS microbiology letters, 277(1), 50-55 (2007-11-08)
Haloferax mediterranei is a halophilic archaeon that can grow using nitrate as the sole nitrogen source. A ferredoxin that serves as the physiological electron donor to the nitrate and nitrite reductases in this assimilatory process has been characterized. The ferredoxin
Pascal Belin et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(18), 7426-7431 (2009-05-07)
The gene encoding the cytochrome P450 CYP121 is essential for Mycobacterium tuberculosis. However, the CYP121 catalytic activity remains unknown. Here, we show that the cyclodipeptide cyclo(l-Tyr-l-Tyr) (cYY) binds to CYP121, and is efficiently converted into a single major product in

Protocoles

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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