E3406

Anti-EB1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-APC binding protein EB1, Anti-End-Binding-1, Anti-Microtuble-associated protein RP/EB family member 1
• Numéro MDL: MFCD01633308
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 35 kDa
• Espèces réactives: rat, mouse, bovine, pig, canine, human
• Concentration: ~1.5 mg/mL
• Technique(s): immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line; indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2
• Numéro d'accès UniProt: Q15691
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MAPRE1(22919) ; mouse ... Mapre1(13589) ; rat ... Mapre1(114764)

Description

Description générale

Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. EB1 (End-Binding-1) proteins belong to a distinct family of accessory proteins, termed “plus-end tracking proteins” or +TIPs, that bind to the plus-ends of microtubules. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.

Immunogène

synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

Application

Anti-EB1 antibody produced in rabbit has been used in

• immunofluorescence
• western blotting
• immunocytochemistry
• immunoprecipitation

Actions biochimiques/physiologiques

End-Binding-1 (EB1) associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.

The C-terminal domain of End-binding proteins (EBs) is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)