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E1161

Sigma-Aldrich

EDTA Buffer, pH 8.5, 10×, Antigen Retriever

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About This Item

Code UNSPSC :
12352107
Nomenclature NACRES :
NA.47

pH

8.5

Niveau de qualité

Application(s)

hematology
histology

Température de stockage

2-8°C

Application

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8.5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualize antigens by immunohistochemical analysis.

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Expressions of matrix metalloproteinases 2, 7, and 9 in carcinogenesis of pancreatic ductal adenocarcinoma.
Jakubowska K, et al.
Disease Markers, 2016 (2016)
Thomas J Gniadek et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 30(4), 530-538 (2017-01-07)
Predicting response to checkpoint blockade therapy for lung cancer has largely focused on measuring programmed death-ligand 1 (PD-L1) expression on tumor cells. PD-L1 expression is geographically heterogeneous within many tumors, however, and we questioned whether small tissue samples, such as
Hong-Wei Sun et al.
Cancer immunology research, 9(1), 20-33 (2020-11-13)
Metabolism is reprogrammed in cancer to fulfill the demands of malignant cells for cancer initiation and progression. Apart from its effects within cancer cells, little is known about whether and how reprogramed metabolism regulates the surrounding tumor microenvironment (TME). Myeloid-derived

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