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DNASE10

Sigma-Aldrich

On-Column DNase I Digestion Set

sufficient for 10 preparations

Synonyme(s) :

Deoxyribonuclease I Set

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Forme

liquid

Utilisation

sufficient for 10 preparations

Technique(s)

RNA purification: suitable

Température de stockage

−20°C

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Description générale

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit and the Spectrum Plant Total RNA Kit. It can also be used with many other commercially available RNA purification kits.
The vast majority of DNA is eliminated from total RNA purifications by the procedures provided in the standard protocols of the Sigma Kits named below. However, for very sensitive applications, such as quantitative RT-PCR, even trace amounts of DNA can result in false positive detection of cDNA-derived amplicons.

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

The term ′On-Column′ indicates that digestion is accomplished in-process for true convenience. Each of Sigma′s total RNA purification kits utilize a binding column for purification. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow. To review the complete protocol, see the Technical Bulletin for any of the Kits named above.

Application

On-Column DNase I Digestion Set is suitable for removing gDNA from total RNA preparations. It may not be suitable for RT-PCR applications as a small amount of residual DNA may remain. On-Column DNase I Digestion Set was used to purify RNA preparations isolated from Vitis vinifera cultivars.

Caractéristiques et avantages

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

Principe

The on-column digestion is accomplished in-process for convenience. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC
Spectrum is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

RTN350

Kit Miniprep pour ARN total de mammifère GenElute™, sufficient for 350 purifications

RTN70

Kit Miniprep pour ARN total de mammifère GenElute™, sufficient for 70 purifications

RTN10

Kit Miniprep pour ARN total de mammifère GenElute™, sufficient for 10 purifications

DNASE70

On-Column DNase I Digestion Set, sufficient for 70 preparations

STRN10

Kit Spectrum™ pour ARN total de plante, sufficient for 10 purifications

STRN50

Kit Spectrum™ pour ARN total de plante, sufficient for 50 purifications

STRN250

Kit Spectrum™ pour ARN total de plante, sufficient for 250 purifications

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Filipa Monteiro et al.
PloS one, 8(9), e72998-e72998 (2013-09-12)
The pivotal role of cultivated grapevine (Vitis vinifera L.) in many countries economy is compromised by its high susceptibility to Plasmopara viticola, the causal agent of downy mildew disease. Recent research has identified a set of genes related to resistance
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