The concentration of the DNA marker is not part of the specification and that information is not available. Product DPLUS-1K - DirectLoad™ Plus 1kb DNA Ladder is a comparable marker that may be considered an alternative. DPLUS-1KT is noted as having a DNA concentration of 100 ug/ul. The DNA concentration for individual bands is unavailable.
D7058
DirectLoad™ Wide Range DNA Marker
ready-to-use marker for DNA electrophoresis
Synonyme(s) :
DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder, wide range ladder for gel electrophoresis, wide range marker for gel electrophoresis
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1 VIAL
292,00 €
292,00 €
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1 VIAL
292,00 €
About This Item
Code UNSPSC :
41105335
Nomenclature NACRES :
NA.25
292,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Niveau de qualité
Forme
liquid
Utilisation
100 uses
Adéquation
suitable for electrophoresis (DNA)
Température de stockage
−20°C
Catégories apparentées
Description générale
Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Caractéristiques et avantages
- Ready-to-load
- Easy-to-use
- Popular band sizes
Composants
Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Autres remarques
Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Informations légales
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
Produit(s) apparenté(s)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Protocoles
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Contenu apparenté
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Could you provide the concentraion of the marker? Idealy band by band so that it can be used as an estimate of the concentration of samples run along side.
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