Unfortunately, no data for Product D4008's use with flow cytometry application is determined. However, several academic publications associated with this product's use in flow cytometry experiments may provide details that can assist with the experimental design. Please see the link to those publications below:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C26&q=%22Dansylcadaverine%22+%22D4008%22+%22flow+cytometry%22&btnG=
D4008
Dansylcadaverine
≥97% (TLC)
Synonyme(s) :
N-(5-Aminopentyl)-5-dimethylaminonaphthalene-1-sulfonamide, N-(Dimethylaminonaphthalenesulfonyl)-1,5-pentanediamine, Monodansyl cadaverine
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250 MG
72,60 €
100 MG
97,10 €
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584,00 €
72,60 €
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Changer de vue
250 MG
72,60 €
100 MG
97,10 €
1 G
584,00 €
About This Item
Formule empirique (notation de Hill):
C17H25N3O2S
Numéro CAS:
Poids moléculaire :
335.46
Beilstein:
2951070
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352108
ID de substance PubChem :
Nomenclature NACRES :
NA.32
72,60 €
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Devis pour commande en gros
Produits recommandés
Niveau de qualité
Essai
≥97% (TLC)
Forme
powder
Température de stockage
−20°C
Chaîne SMILES
CN(C)c1cccc2c(cccc12)S(=O)(=O)NCCCCCN
InChI
1S/C17H25N3O2S/c1-20(2)16-10-6-9-15-14(16)8-7-11-17(15)23(21,22)19-13-5-3-4-12-18/h6-11,19H,3-5,12-13,18H2,1-2H3
Clé InChI
MLEBFEHOJICQQS-UHFFFAOYSA-N
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Description générale
Dansylcadaverine, also called as monodansylcadaverine (MDC), is a lysosomotropic agent. It is present in acidic and lipid rich regions. Dansylcadaverine also acts as a solvent polarity probe.[1] It inhibits vesicular transport and decreases the rotavirus sequestering in L cells, when pre-treated.[2]
Application
Dansylcadaverine in methanol has a reported λex of 335 nm and λem of 512 nm. It has been used:
- as an endocytosis inhibitor in human proximal tubular HK-2 cells,(62) mouse J774A.1 macrophages and human A549 epithelial cells(63)
- to label autophagic vacuoles in cardiomyocytes for fluorescence microscopy studies(64)
- in monodansylcadaverine (MDC) staining of fibroblasts in Ankylosing spondylitis (AS) samples(65)
Actions biochimiques/physiologiques
The autofluorescent dye dansylcadaverine, or monodansylcadaverine (MDC), is commonly used to monitor autophagy. MDC preferentially accumulates in autophagic vacuoles due to a combination of ion trapping and specific interactions with membrane lipids.
Conditionnement
Bottomless glass bottle. Contents are inside inserted fused cone.
air sensitive, handled under inert gas
Autres remarques
Solubility of dansylcadaverine is tested in methanol (10 mg/mL). It has also been reported that dansylcadaverine is soluble in DMSO and acetic acid.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Gloves
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Les clients ont également consulté
Dansylcadaverine and cytochalasin D enhance rotavirus infection of murine L cells
Bass DM, et al.
Virology, 212(2), 429-437 (1995)
Fluorescence properties and staining behavior of monodansylpentane, a structural homologue of the lysosomotropic agent monodansylcadaverine
Niemann A, et al.
The Journal of Histochemistry and Cytochemistry, 49(2), 177-185 (2001)
Shih T Khew et al.
Biomaterials, 31(16), 4600-4608 (2010-03-13)
Transglutaminase (TGase)-modified proteins are commonly observed in a wide range of biological systems. Therefore, the identification of TGase substrates and respective consensus sites may contribute to a better understanding of the physiological role of TGase. In this study, we identified
P J Davies et al.
Diabetes care, 7 Suppl 1, 35-41 (1984-05-01)
It has been suggested that cellular transglutaminases may play an important role in the process of receptor-mediated endocytosis. We have examined this premise by investigating the receptor-mediated endocytosis of two proteins, epidermal growth factor and alpha 2 macroglobulin in cells
Raúl A Almeida et al.
Veterinary research communications, 35(2), 89-101 (2011-01-06)
Research on mastitis in dairy cows caused by Escherichia coli has reported the emergence of strains capable of inducing chronic mastitis and that these strains adhered to and internalized into bovine mammary epithelial cells better than strains of E. coli
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Can I use Dansylcadaverine D4008 for detection of autophagy using flow cytometry? if Yes which laser should we use to detect the MDC signal? Di you have any recommendations regarding the final concentration of MDC for detection of autophagy in lymphocyte?
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Can Dansylcadaverine be use as a marker of autophagic vacuoles?
1 answer-
Dansylcadaverine is a lysosomotropic compound that can be used for the identification of autophagic vacuoles. Please refer to the literature for application-specific protocols.
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What is Product D4008, Dansylcadaverine, soluble in?
1 answer-
We test the solubility of this product in acetic acid at a concentration of 50 mg/mL, with the application of heat and sonication. It has also been reported as being soluble in methanol and DMSO. We have not validated this claim in our labs.
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What is the solution stability of Product D4008, Dansylcadaverine?
1 answer-
According to one source a stock solution of monodansylcadaverine was prepared in methanol at 50 mM and stored frozen at -20°C. No time frame for long-term solution stability was given.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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