Yes, this material has a fluorescence spectrum similar to DAPI, with excitation at 340-380 nm and emission at 385-470 nm. The images displayed above were acquired using UV filter set (340-380 nm excitation, 40 nm dichroic, 430-nm long pass filter). Please note that Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately.
SAE0087
Filipin III ready made solution
from Streptomyces filipinensis, 1mg/ml in DMSO based solution
Synonyme(s) :
Filipin III solution
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
1 ML
295,00 €
295,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Sélectionner une taille de conditionnement
Changer de vue
1 ML
295,00 €
About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77
295,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Niveau de qualité
Essai
≥95% (HPLC)
Forme
ready-to-use solution
Conditions de stockage
protect from light
Conditions d'expédition
dry ice
Température de stockage
−20°C
Catégories apparentées
Description générale
Filipin III is a polyene macrolide antibiotic and the major component isolated from cultures of S. filipinensis. 1 Due to its ability to fluoresce and bind to cholesterol, Filipin has been widely
used as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for free cholesterol in the study of Type C Niemann-Pick disease. 3 Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. 4 Filipin III was found to trigger signaling responses in tobacco cells, including a NADPH oxidase-dependent production. 5. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
used as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for free cholesterol in the study of Type C Niemann-Pick disease. 3 Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. 4 Filipin III was found to trigger signaling responses in tobacco cells, including a NADPH oxidase-dependent production. 5. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
Application
Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.
Autres remarques
- Filipin III solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C avoid freeze thaw cycles.
- Filipin III is used in various concentrations depending on the specific protocol used. Dilute 1mg/ml stock solution with appropriate buffer according to the used protocol.
- Filipin III interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
188.6 °F
Point d'éclair (°C)
87 °C
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Yun-Qi Zhou et al.
Emerging microbes & infections, 11(1), 1135-1144 (2022-03-29)
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants is threatening public health around the world. Endocytosis functions as an important way for viral infection, and SARS-CoV-2 bears no exception. However, the specific endocytic mechanism of
Pan Gao et al.
Investigative ophthalmology & visual science, 63(5), 32-32 (2022-05-27)
Bietti crystalline dystrophy (BCD) is a progressive retinal degenerative disease primarily characterized by numerous crystal-like deposits and degeneration of retinal pigment epithelium (RPE) and photoreceptor cells. CYP4V2 (cytochrome P450 family 4 subfamily V member 2) is currently the only disease-causing
M A Castanho et al.
The Journal of biological chemistry, 267(1), 204-209 (1992-01-05)
The alterations in the absorption and fluorescence spectra observed for the polyene antibiotics filipin and nystatin in the presence of cholesterol are due to an exciton interaction (polyene aggregates) and cannot be attributed to a specific sterol-antibiotic complex. Filipin and
Nina H Pipalia et al.
Journal of lipid research, 47(2), 284-301 (2005-11-17)
Niemann-Pick disease type C (NPC) is an autosomal recessive genetic disorder manifested by abnormal accumulation of unesterified cholesterol and other lipids. We screened combinatorially synthesized chemical libraries to identify compounds that would partially revert cholesterol accumulation. Cultured CHO cells with
Ruochen Zang et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(50), 32105-32113 (2020-11-27)
Cholesterol 25-hydroxylase (CH25H) is an interferon (IFN)-stimulated gene that shows broad antiviral activities against a wide range of enveloped viruses. Here, using an IFN-stimulated gene screen against vesicular stomatitis virus (VSV)-SARS-CoV and VSV-SARS-CoV-2 chimeric viruses, we identified CH25H and its
-
Would it be possible to image this product using a DAPI filter (Excitation 377 nm, Emission 447 nm)?
1 answer-
Helpful?
-
-
Any suggestions for addressing white precipitations observed during Filipin staining for endothelial cells, possibly due to DMSO? Also, seeking advice to improve the experiment where most cells were negative for Filipin staining, and only a few were positive while measuring cholesterol content in HUVEC cells.
1 answer-
To address white precipitations observed during Filipin staining for endothelial cells, increasing the amount of DMSO in the final staining solution may be necessary. This can be achieved by diluting the 1 mg/ml Filipin III solution with more DMSO solution to create a 0.5 mg/ml or 0.1 mg/ml concentrated solution prior to staining the samples.
For improving cholesterol content measurement in HUVEC cells, optimize the working concentration of Filipin and conduct a time-course experiment to enhance staining results. Prompt analysis of stained samples can help prevent photobleaching.Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
