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CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Synonyme(s) :

Mitochondrial activity assay

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Conditionnement

pkg of 1 kit

Technique(s)

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

Application(s)

cell analysis
detection

Méthode de détection

colorimetric

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Conditionnement

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Pictogrammes

FlameHealth hazardExclamation mark
GHS02,GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319,H336,H341

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

Organes cibles

Central nervous system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

53.6 °F - closed cup

Point d'éclair (°C)

12 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Abdullah Maha et al.
Hematology (Amsterdam, Netherlands), 13(1), 13-20 (2008-06-07)
Despite the advances in understanding the pathophysiology of acute myeloid leukaemia (AML), the cure rate for acute myeloid leukaemia patients remains low. Cytogenetic abnormalities and age are the prognostic factors that guide treatment decisions. However, many AML patients still die.
Cassandra D Kelly-Cirino et al.
Infection and immunity, 77(11), 4859-4867 (2009-08-26)
The anthrax protective antigen (PA) is the receptor-binding subunit common to lethal toxin (LT) and edema toxin (ET), which are responsible for the high mortality rates associated with inhalational Bacillus anthracis infection. Although recombinant PA (rPA) is likely to be
Carla M R Lacerda et al.
American journal of physiology. Heart and circulatory physiology, 302(10), H1983-H1990 (2012-02-22)
This study addressed the following questions: 1) Does cyclic tensile strain induce protein expression patterns consistent with myxomatous degeneration in mitral valves? 2) Does cyclic strain induce local serotonin synthesis in mitral valves? 3) Are cyclic strain-induced myxomatous protein expression
Qiuyu Lin et al.
Journal of cellular and molecular medicine, 22(10), 4640-4652 (2018-07-25)
This study is aimed to investigate the methylation level of candidate genes and its impact on thyroid carcinoma (THCA) development. Infinium Human Methylation 450 BeadChip Arrays by Illumina (Illumina HM450K) was the most popular CpG microarray platform widely used in
Ahran Pae et al.
The journal of advanced prosthodontics, 6(2), 96-102 (2014-05-21)
This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate
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Quality Control Considerations in Cell Culture

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