PEA-13

97040311, mouse embryo, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: PEA-13, 97040311
• Source biologique: mouse embryo
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse embryo fibroblast, LRP-deficient, homozygous

Description de la lignée cellulaire

The cell line PEA-13 is homozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-13 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Pseudomonas exotoxin A (PEA) was used to select murine fibroblasts genetically deficient in LRP. Together with the cell lines PEA-10 (Sigma Catalogue number. 97040310) and MEF-1 (Sigma Catalogue number. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP-deficiency. This is of importance since LRP-deficient mouse embryos die early during gestation.

Application

Study of LRP and LRP deficiency.

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.