C9282

Cholate de sodium hydrate

BioReagent, suitable for cell culture

• Synonyme(s) : Acide 3α,7α,12α-trihydroxy-5β-cholan-24-oïque sodium salt, Acide cholalique sodium salt
• Formule empirique (notation de Hill) : C₂₄H₃₉NaO₅ · xH₂O
• Numéro CAS: 206986-87-0
• Poids moléculaire : 430.55 (anhydrous basis)
• Beilstein: 3582354
• Numéro CE : 206-643-5
• Numéro MDL: MFCD00150749
• Code UNSPSC : 12161902
• ID de substance PubChem : 24893147
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: bovine bile; ovine bile
• Description: anionic
• Gamme de produits: BioReagent
• Essai: ≥99.0% (HPLC)
• Forme: powder
• Poids mol.: micellar avg mol wt 900-1300
• Nombre d'agrégation: 2-3
• Technique(s): cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• Solubilité: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• Chaîne SMILES: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1
• Clé InChI: MUVVIYFKOVLQHL-RCVKHMDESA-M

Description

Description générale

Cholate is a primary bile acid[1] produced from cholesterol in the liver.[2] It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).[3]

Application

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system[4],
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor[5]

Actions biochimiques/physiologiques

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.[2]

Informations sur la sécurité

• Mentions de danger: H412
• Conseils de prudence: P273 - P501
• Classification des risques: Aquatic Chronic 3
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)