C1427

Cytochrome b₅ human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

• Synonyme(s) : Cytochrome b5
• Numéro MDL: MFCD02687283
• Code UNSPSC : 12161501
• Nomenclature NACRES : NA.47

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli (histidine-tagged)
• Pureté: ≥90% (SDS-PAGE)
• Forme: solution
• Poids mol.: 16 kDa
• Technique(s): inhibition assay: suitable
• Adéquation: suitable for molecular biology
• Numéro d'accès UniProt: P00167
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CYB5A(1528)

Description

Description générale

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Actions biochimiques/physiologiques

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Forme physique

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Remarque sur l'analyse

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)