Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
A6559
Adenosine 5′-triphosphate disodium salt solution
Crystalline ATP, HPLC purified, aqueous solution for RNA transcription
Synonyme(s) :
ATP
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
25 μMOL
175,00 €
175,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Sélectionner une taille de conditionnement
Changer de vue
25 μMOL
175,00 €
About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
41106305
eCl@ss :
32160414
ID de substance PubChem :
Nomenclature NACRES :
NA.52
175,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Niveau de qualité
Forme
liquid
Concentration
100 mM in H2O (adjusted to pH 7 with Trizma®
base)
Activité étrangère
DNase, RNase, none detected
Conditions d'expédition
dry ice
Température de stockage
−20°C
Chaîne SMILES
[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1
Clé InChI
TTWYZDPBDWHJOR-IDIVVRGQSA-L
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Catégories apparentées
Description générale
Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.
Application
Adenosine 5′-triphosphate disodium salt solution has been used:
- as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular[1]
- as a component of the internal pipette solution to establish a recording electrode to study neurite stretching[2]
- in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay[3]
- as a component of lysis and resealing buffers for ATP studies[4]
- in spectrophotometric measurement of H+ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells[5]
- to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)[6]
Actions biochimiques/physiologiques
Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.[7]
Informations légales
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Cooper GM
The Cell: A Molecular Approach (2000)
Identification of small molecule inhibitors of phosphatidylinositol 3-kinase and autophagy.
Farkas T
The Journal of Biological Chemistry, 286(45), 38904-38912 (2011)
Shaojian Chen et al.
Cell cycle (Georgetown, Tex.), 20(12), 1107-1121 (2021-06-08)
Studies have found that cell pyroptosis is involved in the occurrence and development of rheumatoid arthritis (RA). Hsa_circ_0044235 has been found to be significantly low-expressed in RA patients. The purpose of this research was to reveal the regulatory mechanism of
Yahui Huang et al.
Archiv der Pharmazie (2018-06-29)
Inhibition of spleen tyrosine kinase (Syk) is a promising strategy for the treatment of various allergic and autoimmune disorders such as asthma, rheumatoid arthritis, and allergic rhinitis. Previously, a Syk inhibitor with novel indazole scaffold was discovered by structure-based virtual
James Franklin Tucker et al.
PLoS genetics, 12(2), e1005873-e1005873 (2016-02-20)
Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including
Articles
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
Comprendre comment les vaccins à ARNm induisent l'immunité et la façon dont l'ARNm de synthèse est préparé pour les vaccins immunogènes et autres produits biopharmaceutiques. Trouver des réactifs pour la synthèse de l'ARNm.
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used in molecular biology applications?
1 answer-
This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.
Helpful?
-
-
What is the millimolar extinction coefficient for ATP, Product No. A6559?
1 answer-
The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-
-
Can this preparation of Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used as an ATP standard?
1 answer-
This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).
Helpful?
-
-
What is meant by 25UMO in the size description of Product A6559, Adenosine 5´-triphosphate disodium salt solution? What is the product dissolved in?
1 answer-
25UMO means 25 micromoles. The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution pH has been adjusted with Trizma base but contains no additional buffer or salts.
Helpful?
-
-
What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?
1 answer-
ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25. The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration. For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.
Helpful?
-
-
Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used for in vitro transcription?
1 answer-
The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.
Helpful?
-
-
How long can aqueous ATP solutions like Product No. A6559 be stored?
1 answer-
Aqueous solutions of ATP are stable for months when frozen in solution at -15 °C and for approximately one week at 0 °C. As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
