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04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Synonyme(s) :

Staining kit for live/dead cells

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1 KIT
1 120,00 €

1 120,00 €

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1 KIT
1 120,00 €

About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32

1 120,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Adéquation

suitable for fluorescence

Niveau de qualité

100

Température de stockage

−20°C

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Composants de kit seuls

Réf. du produit
Description
  • Solution A (Calcein AM solution) 4 × 50
  • Solution B (propidium iodide solution) 300 μL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

92210

Timestrip Plus™ 0 °C

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

185.0 °F - closed cup

Point d'éclair (°C)

85 °C - closed cup

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Certificats d'analyse (COA)

Lot/Batch Number
BCCM1249
BCCK6792
BCCJ1064
BCCH3243
BCCG3361

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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T Kimura et al.
Neuroscience letters, 208(1), 53-56 (1996-04-12)
The recent immunological demonstration of advanced glycation end products (AGE) of the Maillard reaction in several human tissues suggests a possible involvement of AGE in the aging process. We previously prepared a monoclonal anti-AGE antibody (6D12) which recognized N epsilon-(carboxymethyl)lysine.
T Meshulam et al.
The Journal of infectious diseases, 172(4), 1153-1156 (1995-10-01)
Studies of antimycotic host defenses have been limited by the paucity of rapid, reproducible quantitative assays for fungal cell damage. Prior studies defined a colorimetric method that uses MTT, a tetrazolium dye, to quantify polymorphonuclear leukocyte (PMNL)-mediated damage to fungi.
T Matsuse et al.
Journal of clinical pathology, 51(7), 515-519 (1998-11-03)
To investigate the presence and distribution of advanced glycation end products (AGE) in pulmonary fibrosis. Lung tissue samples obtained from seven necropsy cases with idiopathic pulmonary fibrosis and seven with normal pulmonary parenchyma were examined immunohistochemically with a monoclonal antibody
Richardson N Leão et al.
PloS one, 5(11), e13833-e13833 (2010-11-17)
Pluripotent and multipotent stem cells hold great therapeutical promise for the replacement of degenerated tissue in neurological diseases. To fulfill that promise we have to understand the mechanisms underlying the differentiation of multipotent cells into specific types of neurons. Embryonic
Zhen Cao et al.
Cellular and molecular neurobiology, 35(8), 1073-1079 (2015-07-03)
Scorpion venom has been used in the Orient to treat central nervous system diseases for many years, and the protein/peptide toxins in Buthus martensii Karsch (BmK) venom are believed to be the effective components. Scorpion venom heat-resistant peptide (SVHRP) is
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Questions

1–3 of 3 Questions  

  1. Can this kit be used to assess cell viability within bacterial biofilms?

    1 answer

    1. This product requires preparation of a cell suspension and has not been validated for bacterial biofilms or cells attached to any surface.

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  2. Can the live/dead double staining kit be used on fixed cells?

    1 answer

    1. The kit is not designed for use with just dead cells. When cells are fixed, it typically results in cell death for all cells. Consequently, if all the cells are dead, it is expected that the Calcein AM would not be able to enter the cells, as Calcein AM is not a fluorescent molecule. The available data sheet states, "While the cells are viable, the calcein generated from CalceinAM by esterase in a viable cell emits strong green fluorescence (excitation: 490 nm, emission: 515 nm)." Therefore, Calcein-AM only stains viable cells. If all the cells are dead, the only observed staining will be from the Propidium Iodide - which is the dead cell stain reagent in kit 04511.
      If the cells are stained while viable, it is possible to image the cells after they have been fixed in paraformaldehyde or other fixatives. Although this is a common practice in some laboratories, It's suggested to consider a 10-minute fixation instead of 30 minutes, as fixing for 30 minutes may not be advisable.

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  3. How many assays or tests can be conducted using one kit of PN: 04511-1KT-F?

    1 answer

    1. The 04511 kit consists of 2 components, with reagent B being the limiting reagent, requiring just 300 microliters of reagent. To prepare the staining solution, 10 microliters of Reagent A and 5 microliters of Reagent B are mixed with 5 ml of PBS. Each test necessitates 100 microliters of the staining solution. With 5 microliters of Reagent B being sufficient for 50 tests, 300 microliters of Reagent B is adequate for 60 batches of the staining reagent, resulting in 3000 tests (50 x 60). Although the maximum number of specimens might not always be stained in practice, the kit theoretically should allow for 3000 tests.

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