G418-RO

G 418 disulfate salt solution

solution, =98% (TLC), suitable for transfection

• Synonyme(s) : Antibiotique G418
• Formule empirique (notation de Hill): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• Numéro CAS: 108321-42-2
• Poids moléculaire : 692.71
• Numéro MDL: MFCD00058314
• Code UNSPSC : 12352200
• ID de substance PubChem : 329799925
• Nomenclature NACRES : NA.28

Propriétés

• Niveau de qualité: 100
• Pureté: 98% (TLC)
• Forme: solution
• Poids mol.: 692.7 Da
• Conditionnement: pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g]); pkg of 20 mL (04727878001 [equivalent to 1 g])
• Fabricant/nom de marque: Roche
• Concentration: 50 mg/mL
• Technique(s): transfection: suitable
• Spectre d'activité de l'antibiotique: protozoa
• Mode d’action: protein synthesis | inhibits
• Température de stockage: 2-8°C
• Chaîne SMILES: OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• Clé InChI: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

Description générale

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

Application

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neo^r) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Actions biochimiques/physiologiques

Mode d′action : G418 bloque la synthèse des polypeptides et l′élongation des protéines en inhibant la synthèse au niveau des ribosomes 70S et 80S.

Spectre antimicrobien : G418 a permis la sélection de cellules transfectées de façon stable par un plasmide contenant le promoteur iNOS et le gène de résistance à la néomycine ; cet antibiotique présente également une activité contre les protozoaires et helminthes.

Caractéristiques et avantages

• Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
• Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
• Function tested in a cell culture assay - ensures effectiveness.
• Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
• Cost effective, since the selected colonies are stable.

Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Qualité

Purity: = 98%, as shown by TLC.
Function testing: in cell culture.

Notes préparatoires

Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317 - H334
• Conseils de prudence: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available