11796895001
Roche
DIG Easy Hyb™ Granules
powder, suitable for hybridization
Synonyme(s) :
DIG system
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About This Item
Code UNSPSC :
41105326
Produits recommandés
Agence
suitable for
Niveau de qualité
Forme
powder
Utilisation
sufficient for 6 x 100 mL
Fabricant/nom de marque
Roche
Conditions de stockage
(Keep container tightly closed in a dry and well-ventilated place.)
Caractéristiques du produit alternatif plus écologique
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Technique(s)
hybridization: suitable
Couleur
white
pH
7,0
Solubilité
water: soluble
Adéquation
suitable for nucleic acid hybridization
Autre catégorie plus écologique
Température de stockage
20-25°C
Description générale
DIG Easy Hyb™ is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.
Caractéristiques et avantages
- DIG Easy Hyb™ is a convenient ready-to-use hybridization buffer, saving time and effort.
- DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.
Notes préparatoires
Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Informations légales
DIG Easy Hyb is a trademark of Roche
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Protocoles
Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting
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