Accéder au contenu
Merck
Toutes les photos(1)

Documents

11633716001

Roche

Anti-Digoxigenin-POD (poly), Fab fragments

from sheep

Synonyme(s) :

anti-digoxigenin, digoxigenin

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352203

Source biologique

sheep

Niveau de qualité

100

Conjugué

peroxidase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized

Conditionnement

pkg of 50 U

Fabricant/nom de marque

Roche

Isotype

IgG

Température de stockage

2-8°C

Catégories apparentées

Description générale

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Spécificité

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Application

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
  • whole-mount in situ hybridization
  • fluorescence in situ hybridization
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • Southern blot
  • western blot
  • double labeling experiments
  • lectin blots

Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

Notes préparatoires

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • Dot blot: 50 to 100 mU/ml
  • ELISA: 2 to 50 mU/ml
  • Western blot: 50 to 100 mU/ml
  • Southern blot: 50 to 100 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Reconstitution

Add 1 ml double-distilled water to a final concentration of 50 U/ml.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Not finding the right product?  

Try our Outil de sélection de produits.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Christopher R Campos et al.
PloS one, 15(3), e0229850-e0229850 (2020-03-11)
Reducing Amyloid β (Aβ) in the brain is of fundamental importance for advancing the therapeutics for Alzheimer`s disease. The endogenous metallopeptidase neprilysin (NEP) has been identified as one of the key Aβ-degrading enzymes. Delivery of NEP to the brain by
Potent and selective BACE-1 peptide inhibitors lower brain Abeta levels mediated by brain shuttle transport
<BIG>Ruderisch N, et al.</BIG>
EBioMedicine, 24, 76-92 (2017)
Louise Heydenreich Jensen et al.
International archives of allergy and immunology, 178(4), 307-314 (2019-02-14)
Infant formulas (IFs) based on hydrolysed cow's milk proteins are central in the management of cow's milk allergy (CMA) in infants and small children. New IF compositions with improved prevention and treatment properties are needed, along with appropriate preclinical animal
A quantitative study of the diversity of stripe-forming processes in an arthropod cell-based field undergoing axis formation and growth
<BIG>Hemmi N, et al.</BIG>
Developmental Biology Journal, 437, 84-104 (2018)
RNA Detection and Visualization Methods and Protocols (2011)
Afficher tous les articles scientifiques apparentés

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique