11412272001

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

• Synonyme(s) : hTGF
• Code UNSPSC : 12352207

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in CHO cells
• Essai: >95% (SDS-PAGE)
• Forme: solution
• Puissance: <0.1 ng/mL EC₅₀
• Poids mol.: 25,000 Da
• Conditionnement: pkg of 1 mL (1 μg)
• Fabricant/nom de marque: Roche
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: <0.1 EU/μgtested (LAL test)
• Numéro d'accès UniProt: P01137
• Température de stockage: −20°C
• Informations sur le gène: human ... TGFB1(7040)

Description

Description générale

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Spécificité

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC₅₀: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Application

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.[1]

Actions biochimiques/physiologiques

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.[2]

Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.[3]

Qualité

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Séquence

Chain Length 2 x 112 AA

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Définition de l'unité

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Notes préparatoires

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash