10105031001

β-Galactosidase

from E. coli overproducer

• Synonyme(s) : β-galactosidase
• Numéro de classification (Commission des enzymes): 3.2.1.23 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: Escherichia coli
• Niveau de qualité: 100
• Forme: suspension
• Poids mol.: 540 kDa
• Conditionnement: pkg of 1,500 U
• Fabricant/nom de marque: Roche
• Concentration: 5.00 mg/mL
• Technique(s): activity assay: suitable
• pH optimal: 7
• Adéquation: suitable for UV spectrophotometry and general use
• Application(s): life science and biopharma
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

β-Galactosidase or β-D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K⁺ or Na⁺ and Mg²⁺ to be fully active. β-Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with β-d-galactopyranosides.[1]

At 37 °C with 2-nitrophenyl-β-D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.

Spécificité

Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.

Application

β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA).[2] Use β-Galactosidase to produce a calibration curve in enzymatic assays.

Qualité

Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each

Forme physique

Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline

Notes préparatoires

The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.

In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg²⁺ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.

Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available