MABC570

Anti-Sec8 Antibody/Exoc4, clone 2E12

clone 2E12, from mouse

• Synonyme(s) : Exocyst complex component 4, Exocyst complex component Sec8, rSec8
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2E12, monoclonal
• Espèces réactives: human, rat, mouse
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_446327
• Numéro d'accès UniProt: Q62824
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EXOC4(60412)

Description

Description générale

Sec8/Exoc4 is a component of the exocyst complex which is a multi-protein complex used for docking of exocytic vesicles with fusion sites on the plasma membrane. The exocyst complex is essential for exocytosis which is the process whereby proteins, hormones, transmitters etc. are transported and released to the extracellular environment. The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Sec8/Exoc4 appears to be important for tethering of secretory vesicles to specific regions on the plasma membrane. It seems particularly important in normal development for growth and morphological change by allowing the secretion of matrix metalloproteinases such as MMP2 or MMP9 in discrete locations. Knockdowns exhibit reduced cellular proliferation as well as invasive activities as well. As such Sec8/Exoc4 is important in tumor growth and invasion as well, thus it is also a current target for cancer therapy.

Immunogène

Recombinant protein corresponding to rat Sec8/Exoc4.

Application

Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sec8/Exoc4 in HeLa, A431, and NIH/3T3 cells. DyLight^® is a registered trademark of Thermo Fisher Scientific.
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells treated with EXO70 SiRNA (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in B16F1 cells treated with siRNA targeting Sec8 (Zhao, Y., et al. (2013). Dev. Cell. 26:266-278).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells transfected with GFP-Exo70 (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in Rat emreyonic hippocampal neurons (Menchon, S.A., et al. (2011). PLOS ONE. 6(9):e24190 1-11).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Sec8 Antibody/Exoc4, clone 2E12 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sec8/Exoc4.

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sec8/Exoc4 in 10 µg of HeLa cell lysate.

Description de la cible

~110 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

DyLight is a registered trademark of Pierce Biotechnology, Inc.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable