506123
Anti-p38 MAP Kinase (341-360) Rabbit pAb
liquid, Calbiochem®
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
liquid
Ne contient pas
preservative
Espèces réactives
mouse, rat, human
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Mapk11(19094)
Description générale
Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~38 kDa p38 MAPK protein.
Recognizes the ~38 kDa p38 MAPK protein.Antibody Target Gene Symbol: MAPK14 Target Synonym: CRK1, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Hog, MAPK p38, MGC102436, MGC105413, MXI2, P38, P38 KINASE, P38 Map Kinase, p38 Mapk alpha, P38-ALPHA, p38-RK, p38/Hog1, p38/Mpk2, P38/RK, p38a, p38Hog, p38MAPK, PRKM14, PRKM15, RK, SAPK2A Entrez Gene Name: mitogen-activated protein kinase 14 Hu Entrez ID: 1432 Mu Entrez ID: 26416 Rat Entrez ID: 81649
This Anti-p38 MAP Kinase (341-360) Rabbit pAb is validated for use in Flow Cytometry, Immunoblotting, Paraffin Sections for the detection of p38 MAP Kinase (341-360).
Immunogène
Human
a synthetic peptide (TYDEYISFVPPPLDQEEMES) corresponding to amino acids 341-360 of human p38 MAP kinase
Application
Flow Cytometry (1:25)
Immunoblotting (1:1000)
Paraffin Sections (1:50, heat pretreatment required, see comments)
Immunoblotting (1:1000)
Paraffin Sections (1:50, heat pretreatment required, see comments)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 150 mM NaCl, 10 mM HEPES, 50% glycerol, 0.01% BSA, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Autres remarques
Pretreat paraffin sections by heating tissue in 10 mM citrate buffer, pH 6.0 for 1 min at high power followed by 9 min at medium power; keep the slides fully immersed and maintain the temperature at or just below boiling; cool the slides for 20 min at room temperature prior to staining. Variables associated with assay conditions will dictate the proper working dilution.
Recommended Protocol for Immunoblotting
Solutions and Reagents
• Transfer Buffer: 25 mM Tris base, 0.2 M glycine, 20% methanol, pH 8.5.
• SDS Sample Buffer: 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50 mM DTT, 0.1% bromophenol blue.
• 10X TBS (Tris-buffered saline): To prepare 1 liter, 24.2 g Tris base, 80 g NaCl, adjust pH to 7.6 with HCl. Dilute 1:10 for use.
• Blocking Buffer: 1X TBS, 0.1% Tween®-20 detergent with 5% non-fat dry milk.
• Primary Antibody Dilution Buffer: 1X TBS, 0.1% Tween-20 detergent with 5% BSA
• Wash Buffer (TBST): 1X TBS, 0.1% Tween-20 detergent
Blotting Membrane
Nitrocellulose or PVDF membranes may be used.
Protein Blotting
1. Lyse cells by adding 100 ml SDS Sample Buffer and immediately scrape the cells off the plate and transfer the extract to a microfuge tube. Keep on ice.
2. Sonicate for 2 s to shear DNA and reduce sample viscosity.
3. Heat sample to 95-100°C for 5 min. Cool on ice.
4. Microcentrifuge for 5 min.
5. Load 20 ml onto SDS-PAGE gel (10 cm x 10 cm).
6. Electrotransfer to nitrocellulose membrane.
As controls, we recommend using 15 ml of phosphorylated and nonphosphorylated C-6 glioma cell extracts.
Membrane Blocking, Gel and Antibody Incubations
1. After transfer, wash membrane with 25 ml TBS for 5 min at room temperature.
2. Incubate membrane in 25 ml of Blocking Buffer for 1-3 h at room temperature or overnight at 4°C.
3. Wash 3 times for 5 min each with 15 ml TBST.
4. Incubate membrane and primary antibody (at the appropriate dilution) in 10 ml Primary Antibody Dilution Buffer with gentle agitation overnight at 4°C.
5. Wash 3 times for 5 min each with 15 ml TBST.
6. Incubate membrane with conjugated secondary antibody at the appropriate dilution in 10 ml Blocking Buffer with gentle agitation for 1 h at room temperature.
7. Wash membrane as in step 5.
Detection of Proteins
Chemiluminescence.
Recommended Protocol for Immunoblotting
Solutions and Reagents
• Transfer Buffer: 25 mM Tris base, 0.2 M glycine, 20% methanol, pH 8.5.
• SDS Sample Buffer: 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50 mM DTT, 0.1% bromophenol blue.
• 10X TBS (Tris-buffered saline): To prepare 1 liter, 24.2 g Tris base, 80 g NaCl, adjust pH to 7.6 with HCl. Dilute 1:10 for use.
• Blocking Buffer: 1X TBS, 0.1% Tween®-20 detergent with 5% non-fat dry milk.
• Primary Antibody Dilution Buffer: 1X TBS, 0.1% Tween-20 detergent with 5% BSA
• Wash Buffer (TBST): 1X TBS, 0.1% Tween-20 detergent
Blotting Membrane
Nitrocellulose or PVDF membranes may be used.
Protein Blotting
1. Lyse cells by adding 100 ml SDS Sample Buffer and immediately scrape the cells off the plate and transfer the extract to a microfuge tube. Keep on ice.
2. Sonicate for 2 s to shear DNA and reduce sample viscosity.
3. Heat sample to 95-100°C for 5 min. Cool on ice.
4. Microcentrifuge for 5 min.
5. Load 20 ml onto SDS-PAGE gel (10 cm x 10 cm).
6. Electrotransfer to nitrocellulose membrane.
As controls, we recommend using 15 ml of phosphorylated and nonphosphorylated C-6 glioma cell extracts.
Membrane Blocking, Gel and Antibody Incubations
1. After transfer, wash membrane with 25 ml TBS for 5 min at room temperature.
2. Incubate membrane in 25 ml of Blocking Buffer for 1-3 h at room temperature or overnight at 4°C.
3. Wash 3 times for 5 min each with 15 ml TBST.
4. Incubate membrane and primary antibody (at the appropriate dilution) in 10 ml Primary Antibody Dilution Buffer with gentle agitation overnight at 4°C.
5. Wash 3 times for 5 min each with 15 ml TBST.
6. Incubate membrane with conjugated secondary antibody at the appropriate dilution in 10 ml Blocking Buffer with gentle agitation for 1 h at room temperature.
7. Wash membrane as in step 5.
Detection of Proteins
Chemiluminescence.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Not finding the right product?
Try our Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Xiang-Peng Zeng et al.
Frontiers in pharmacology, 12, 686992-686992 (2021-06-22)
Pancreatic fibrosis is one of the most important pathological features of chronic pancreatitis (CP), and pancreatic stellate cells (PSCs) are considered to be the key cells. Puerarin is the most important flavonoid active component in Chinese herb Radix Puerariae, and
Christian C Witt et al.
The EMBO journal, 27(2), 350-360 (2007-12-25)
The muscle-specific RING finger proteins MuRF1 and MuRF2 have been proposed to regulate protein degradation and gene expression in muscle tissues. We have tested the in vivo roles of MuRF1 and MuRF2 for muscle metabolism by using knockout (KO) mouse
Miriam S Giambelluca et al.
Journal of leukocyte biology, 102(3), 829-836 (2017-02-10)
Activation of the adenosine 2A receptor (A2AR) elevates intracellular levels of cAMP and acts as a physiologic inhibitor of inflammatory neutrophil functions. In this study, we looked into the impact of A2AR engagement on early phosphorylation events. Neutrophils were stimulated
Evelyne Peuchant et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(4), 1531-1546 (2017-01-13)
NME1 (nonmetastatic expressed 1) gene, which encodes nucleoside diphosphate kinase (NDPK) A [also known as nonmetastatic clone 23 (NM23)-H1 in humans and NM23-M1 in mice], is a suppressor of metastasis, but several lines of evidence-mostly from plants-also implicate it in
Norika Mengchia Liu et al.
Journal of molecular medicine (Berlin, Germany), 95(3), 335-348 (2016-12-23)
Restenosis after angioplasty is a serious clinical problem that can result in re-occlusion of the coronary artery. Although current drug-eluting stents have proved to be more effective in reducing restenosis, they have drawbacks of inhibiting reendothelialization to promote thrombosis. New
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique