407321-M

γ-Interferon, Rat, Recombinant, E. coli

• Code UNSPSC : 12352202

Propriétés

• Pureté: ≥95% (reversed phase HPLC)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Température de stockage: −20°C

Description

Description générale

Recombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.

Actions biochimiques/physiologiques

Reported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 0.5X PBS, pH 7.2.

Reconstitution

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.

Reconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.

Autres remarques

Alvarado, J., et al. 2005. Immunol. Lett.99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1