203980

α-Bungarotoxin

from Bungarus multicinctus, No contaminants by IEF, lyophilized solid, irreversible binder to motor end-plate acetylcholine receptor, Calbiochem^®

• Synonyme(s) : α-Bungarotoxin, Bungarus multicinctus
• Formule empirique (notation de Hill): C₃₃₈H₅₂₉N₉₇O₁₀₅S₁₁
• Numéro CAS: 11032-79-4
• Poids moléculaire : 7984.12
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• product name: α-Bungarotoxin, Bungarus multicinctus, Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (K_d = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings.
• Niveau de qualité: 100
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: PBS: soluble; water: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

A polypeptide composed of 74 amino acids containing 5 disulfide bridges. Blocks neuromuscular transmission by irreversible binding to the motor end-plate acetylcholine receptor (K_d = 1 nM to 1 pM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced augmentation in intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM). Prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-Bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (K_d = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced increase of intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM), and prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Actions biochimiques/physiologiques

Cell permeable: no

Kd = 1 pM to 1 nM for motor end-plate acetylcholine receptor

Primary Target
Motor end-plate acetylcholine receptor

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Harmful (C)

Séquence

H-Ile-Val-Cys³-His-Thr-Thr-Ala-Thr-Ser-Pro-Ile-Ser-Ala-Val-Thr-Cys¹⁶-Pro-Pro-Gly-Glu-Asn-Leu-Cys²³-Tyr-Arg-Lys-Met-Trp-Cys²⁹-Asp-Ala-Phe-Cys³³-Ser-Ser-Arg-Gly-Lys-Val-Val-Glu-Leu-Gly-Cys⁴⁴-Ala-Ala-Thr-Cys⁴⁸-Pro-Ser-Lys-Lys-Pro-Tyr-Glu-Glu-Val-Thr-Cys⁵⁹-Cys⁶⁰-Ser-Thr-Asp-Lys-Cys⁶⁵-Asn-Pro-His-Pro-Lys-Gln-Arg-Pro-Gly-OH (disulfide bonds: 3 → 23; 16 → 44; 29 → 33; 48 → 59; 60 → 65)

Forme physique

Supplied as an acetate salt

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Remarque sur l'analyse

Contaminants: None detectable by IEF

Autres remarques

Lopez, M.G., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14184.
Zhang, Z.W., et al. 1994. Neuron 12, 167.
Bambrick, L.L., and Gordon, T. 1992. J. Physiol.449, 479.
Lin, S.R., and Chang, C.C. 1992. Biochim. Biophys. Acta1159, 255.
Motomura, M., et al. 1992. Neurosci. Lett.143, 139.
Sorenson, E.M., and Chiappinelli, V.A. 1992. J. Comp. Neurol.323, 1.
Ruan, K.H., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6156.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable