1.01301

Auramine O (C.I. 41000)

for microscopy

• Synonyme(s) : Auramine O (C.I. 41000), Pyoktamin yellow, 4,4′-Bis-(dimethylamino)-benzophenonimide hydrochloride
• Formule empirique (notation de Hill): C₁₇H₂₂ClN₃
• Numéro CAS: 2465-27-2
• Poids moléculaire : 303.83
• Numéro MDL: MFCD00012484
• Code UNSPSC : 41116130
• Numéro de classement CE : 219-567-2

Propriétés

• Niveau de qualité: 400
• Forme: solid
• Puissance: 1000 mg/kg LD₅₀, oral (Rat)
• pH: 6-7 (20 °C, 10 g/L in H₂O)
• Pf: 265 °C (decomposition)
• Solubilité: 10 g/L
• Masse volumique apparente: 430 kg/m³
• εmax: 1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• Clé InChI: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

Description générale

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

Remarque sur l'analyse

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

Informations légales

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07,GHS08,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H302 - H319 - H351 - H411
• Conseils de prudence: P201 - P202 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3