07-645
Anti-Sp1 Antibody
Upstate®, from rabbit
Synonyme(s) :
Sp1 transcription factor, specificity protein 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, human, rat
Conditionnement
antibody small pack of 25 μg
Fabricant/nom de marque
Upstate®
Technique(s)
ChIP: suitable
electrophoretic mobility shift assay: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SP1(6667)
Description générale
Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
Spécificité
Expected to cross-react with rat.
Recognizes Sp1, Mr 105 kDa and 95 kDa.
Immunogène
Full length human Sp1 protein.
Application
Immunoprecipitation:
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com
Chromatin Immunoprecipitation:
Reported by an independent laboratory.
Immunofluorescence:
Reported by an independent laboratory.
Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com
Chromatin Immunoprecipitation:
Reported by an independent laboratory.
Immunofluorescence:
Reported by an independent laboratory.
Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.
Qualité
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Description de la cible
Varies
Liaison
Replaces: MABE550
Forme physique
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Stockage et stabilité
Stable for 1 year at 2-8ºC from date of receipt.
Remarque sur l'analyse
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
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PLoS Genetics null
Yong Wang et al.
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Song, K; Wang, H; Krebs, TL; Wang, B; Kelley, TJ; Danielpour, D
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Constitutive expression of the brg1 gene requires GC-boxes near to the transcriptional start site.
Itoh T, Miyake K, Yamaguchi T, Tsuge M, Kaneoka H, Iijima S
Journal of Biochemistry null
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