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05-584

Sigma-Aldrich

Anti-Tie2/TEK Antibody, clone Ab33

clone Ab33, Upstate®, from mouse

Synonyme(s) :

CD202b antigen, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multip

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

Ab33, monoclonal

Espèces réactives

human, rat, pig, mouse

Fabricant/nom de marque

Upstate®

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NM_000459

Numéro d'accès UniProt

Q02763

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TEK(7010)

Description générale

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Spécificité

Recognizes Tie2/TEK, Mr 145kDa

Immunogène

Fusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33

Application

Immunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory.

Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections.
This antibody is not suitable for paraffin-embedded sections.
This Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK.

Qualité

Evaluated by western blot on RIPA lysates from HUVEC cells.

Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells.

Description de la cible

145 kDa

Forme physique

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Tie2 expression and phosphorylation in angiogenic and quiescent adult tissues.
Wong, A L, et al.
Circulation Research, 81, 567-574 (1997)
Krystyna Teichert-Kuliszewska et al.
Circulation research, 98(2), 209-217 (2005-12-17)
Mutations in the bone morphogenetic protein (BMP) receptor-2 (BMPR2) have been found in patients with idiopathic pulmonary arterial hypertension (IPAH); however, the mechanistic link between loss of BMPR2 signaling and the development of pulmonary arterial hypertension is unclear. We hypothesized
Autocrine role of angiopoietins during megakaryocytic differentiation.
Saulle, E; Guerriero, R; Petronelli, A; Coppotelli, E; Gabbianelli, M; Morsilli et al.
Testing null
Howard Leong-Poi et al.
Circulation research, 101(3), 295-303 (2007-06-23)
Current methods of gene delivery for therapeutic angiogenesis are invasive, requiring either intraarterial or intramuscular administration. A noninvasive method of gene delivery has been developed using ultrasound-mediated destruction of intravenously administered DNA-bearing carrier microbubbles during their microcirculatory transit. Here we
K G Peters et al.
British journal of cancer, 77(1), 51-56 (1998-02-12)
Endothelial receptor tyrosine kinases may play important roles in pathological vascular growth, particularly in tumours. In this study, immunohistochemistry was used to evaluate the expression of a novel endothelial receptor tyrosine kinase, Tie2/Tek, in the endothelium of vascular 'hotspots' in
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