V4505

Monoclonal Anti-Vinculin antibody produced in mouse

clone VIN-11-5, ascites fluid

• Synonyme(s) : Vinculin Antibody Sigma, Anti-VCL
• Numéro MDL: MFCD00162905
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: VIN-11-5, monoclonal
• Poids mol.: antigen 116 kDa
• Espèces réactives: bovine, human, chicken, mouse
• Technique(s): indirect immunofluorescence: 1:50 using cultured chicken fibroblasts; microarray: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: P18206
• Application(s): research pathology
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... VCL(7414) ; mouse ... Vcl(22330)

Description

Description générale

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein.

Spécificité

The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.

Immunogène

smooth muscle vinculin from chicken gizzard.

Application

Monoclonal Anti-Vinculin antibody produced in mouse has been used in:

• western blotting.
• immunofluorescence.
• immunocytochemistry.

Actions biochimiques/physiologiques

Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin α_IIbβ₃ signaling and function, which is required for platelet aggregation.

Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation.

Autres remarques

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable