CC102C

Human Integrin αVβ5 Protein, Triton X-100 Formulation

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Assay: >95% (silver stain, SDS-PAGE)
• form: liquid
• manufacturer/tradename: Chemicon^®
• concentration: 0.325 mg/mL
• NCBI accession no.: NM_002210.2
• UniProt accession no.: P06756
• Gene Information: human ... ITGAV(3685)

Description

General description

Integrin alphaV beta5 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaV beta5 integrin. Two bands are identified with silver stain, corresponding to alphaV (145kDa) and beta5 (90kDa) subunits under nonreducing conditions. Product was tested and found negative for HIV, HBsAg, syphilis, and hepatitis. Integrin alphaV beta5 interacts with vitronectin in ELISA.

Application

Electrophoresis

Immunoblotting (nonreduced conditions)

Ligand Binding studies.

Optimal working dilutions must be determined by end user.

Physical form

Purified protein in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl, 0.2% Triton X-100, with no preservatives.

Storage and Stability

Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles.

Analysis Note

Two main protein bands corresponding to aV (145 kD) and β5 (90 kD) subunits are seen in silver stained gel under non-reduced conditions.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable