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E8909

Sigma-Aldrich

Phosphodiesterase 3B

recombinant, expressed in Sf9 cells

Synonym(s):

3′,5′-cyclic-nucleotide phosphodiesterase, PDE 3B

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in Sf9 cells

Quality Level

form

buffered aqueous solution

specific activity

≥1000 units/μg protein

shipped in

dry ice

storage temp.

−70°C

Application

Phosphodiesterase 3B has been used in a study to evaluate the evidence for biological effects of metformin in operable breast cancer. Phosphodiesterase 3B has also been used in a study to investigate the role of intracellular and intercellular communication in perfusion distribution of erythrocyte-derived ATP.

Physical properties

N-terminal GST-tagged 86 kDa protein containing amino acids 592-end

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cAMP to 5′-AMP per minute at pH 7.4 at 37 °C.

Physical form

Buffered aqueous solution containing Tris-HCl (pH 8.0), NaCl, KCl, Tween-20, DTT, and glycerol

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lin Xie et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 18(4), 444-454 (2011-12-14)
Here we examined whether the expression of a novel immunoregulatory gene set could be used to predict outcomes in murine models of rapamycin-induced cardiac tolerance, spontaneous hepatic tolerance, and cardiac rejection. The expression of the immunoregulatory gene set was assessed
Sirwan Hadad et al.
Breast cancer research and treatment, 128(3), 783-794 (2011-06-10)
Metformin may reduce the incidence of breast cancer and enhance response to neoadjuvant chemotherapy in diabetic women. This trial examined the effects of metformin on Ki67 and gene expression in primary breast cancer. Non-diabetic women with operable invasive breast cancer
Randy S Sprague et al.
Microcirculation (New York, N.Y. : 1994), 19(5), 430-439 (2012-07-11)
In complex organisms, both intracellular and intercellular communication are critical for the appropriate regulation of the distribution of perfusion to assure optimal O(2) delivery and organ function. The mobile erythrocyte is in a unique position in the circulation as it
Koji Ochiai et al.
Bioorganic & medicinal chemistry letters, 23(1), 375-381 (2012-12-04)
(-)-6-(7-Methoxy-2-(trifluoromethyl)pyrazolo[1,5-a]pyridin-4-yl)-5-methyl-4,5-dihydropyridazin-3(2H)-one (KCA-1490) exhibits moderate dual PDE3/4-inhibitory activity and promises as a combined bronchodilatory/anti-inflammatory agent. N-alkylation of the pyridazinone ring markedly enhances potency against PDE4 but suppresses PDE3 inhibition. Addition of a 6-aryl-4,5-dihydropyridazin-3(2H)-one extension to the N-alkyl group facilitates both enhancement
The cyclic AMP pathway.
Paolo Sassone-Corsi
Cold Spring Harbor perspectives in biology, 4(12), doi:10-doi:10 (2012-12-05)

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