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15 mL
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General description
Sigma offers four sizes of gradient mixers. The small, 15 and 30 mL mixers are ideal for the preparation of centrifugation gradients, using sucrose or cesium chloride solutions. The larger, 50 and 100 mL mixers are mainly used for the preparation of linear polyacrylamide gel gradients. The 100 mL mixer is configured to be used in conjunction with the multicaster systems. All of the mixers are produced from a single acrylic block. They consist of two chambers, a reservoir and a mixing chamber, with an interconnecting valve. A second valve regulates the output flow from the mixing chamber. A magnetic stirring bar can be placed in the mixing chamber to maintain a constant gradient. All of the mixers have a flat base to enable them to be placed on a magnetic stirrer. They also have a support rod which can be used to fix the mixers to a stand for extra stability.
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Rawad Hodeify et al.
Science advances, 4(9), eaau1935-eaau1935 (2018-09-29)
Store-operated Ca2+ entry (SOCE) encodes a range of cellular responses downstream of Ca2+ influx through the SOCE channel Orai1. Orai1 recycles at the plasma membrane (PM), with ~40% of the total Orai1 pool residing at the PM at steady state.
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