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biological source
Escherichia coli
Quality Level
form
lyophilized solid
specific activity
≥600 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
water: 2%
shipped in
ambient
storage temp.
−20°C
General description
Native E. coli β-galactosidase. Inducible enzyme not found in eukaryotic cells that is used as a reporter gene. Used to assess efficiency of transfection. Also used in ELISA and immunocytochemical techniques. Histochemical substrates include X-Gal (Cat. No. 203782).
Native E. coli β-galactosidase. Inducible enzyme not found in eukaryotic cells that is used as a reporter gene. Used to assess efficiency of transfection. Also used in ELISA and immunocytochemical techniques. Histochemical substrates include X-Gal (Cat. No. 203782).
Packaging
Yes
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of p-nitrophenyl-β-galactoside per min at 37°C.
Other Notes
Zeleny, R., et al. 1997. Anal. Biochem.246, 96.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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