Skip to Content
Merck

Key Documents

View All Documentation

Skip To

N3262

NCTC 135 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
10 L
Check Cart for Availability
€ 291,00
10 x 1 L
Check Cart for Availability
€ 364,00

About This Item

NACRES:
NA.75
UNSPSC Code:
12171500

€ 291,00

Check Cart for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

shipped in

ambient

storage temp.

2-8°C

General description

NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.

Application

NCTC 135 Medium has been used:
  • to culture Philophthalmus sp. rediae in vitro[1]
  • to culture Ehrlich ascites mouse tumor cell line
  • as a culture media for the differentiation of Rcho-1 cells[2]

Preparation Note

Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
N1140M7403M8537
NCTC 135 Medium With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

SAFC

N3262

NCTC 135 Medium

NCTC 109 Medium With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N1140

NCTC 109 Medium

Sigma-Aldrich

Sigma-Aldrich

M7403

MCDB 153 Medium

MCDB 131 Medium With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M8537

MCDB 131 Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

powder

form

powder

components

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

components

glucose: 1.0 g/L
phenol red: 0.02 g/L
L-glutamine: no
NaHCO3: 2.2 g/L

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

classic media, classical media, FBS, Mouse L-cell culture

mouse L929 cell cultue medium, carcinoma cell line establishment

keratinocye cloning medium, keratinocye long-term culture

HMVEC cloning medium, muscle cell culture medium

Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000448676
0000448672
0000448672
0000448676
25B299

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Related Content

Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Synthesis, characterization and antitumor activity of 1, 2-disubstituted ferrocenes and cyclodextrin inclusion complexes
Petrovski Z, et al.
Journal of Organometallic Chemistry, 693, 675-684 (2008)
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
View All Related Papers

Global Trade Item Number

SKUGTIN
N3262-10X1L04061837459696
N3262-10L04061837459689
N3262-50K04061834182108
N3262-25K04061834182085

Questions

Reviews

No rating value

Active Filters