F6307

FLT1 (784-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): VEGFR1
• NACRES: NA.32
• UNSPSC Code: 51111800
• Form: buffered aqueous glycerol solution
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: ~94 kDa

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 30-40 nmol/min·mg
• mol wt: ~94 kDa
• UniProt accession no.: P17948
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... FLT1(2321)

Description

General description

FLT1 (fms-related tyrosine kinase 1) or VEGFR (vascular endothelial growth factor receptor) is one of the receptor tyrosine kinase, which acts as a receptor for VEGF. VEGF is a predominant angiogenesis stimulating factor. FLT1 is a transmembrane receptor.[1] Soluble FLT1 (sFLT1) is an alternatively spliced soluble form of FLT1, expressed by multiple tissues. It acts as a negative regulator of VEGF. It does not contain the cytosolic and transmembrane domains.[2]

Biochem/physiol Actions

FLT1 (fms-related tyrosine kinase 1) or VEGFR (vascular endothelial growth factor receptor) is implicated in angiogenesis in tumors, which plays a key role in tumorigenesis, invasion and metastasis of tumors, especially the malignant ones.[1] In human circulation, angiogenesis is balanced by soluble FLT1 (sFLT1) and VEGF. An imbalance leads to multiple conditions such as pre-eclampsia.[2] sFLT1 plays an essential role in the angiogenesis of placenta. An overexpression of this protein in placenta is associated with prenatal arsenic exposure and thus, reduced birth weight.[3]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable