C8302

4-Chloro-1-naphthol solution

Name: 4-Chloro-1-naphthol solution
Brand: SIGMA
Price: 109 EUR

For HRP detection on Western Blots

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C8302-100ML

€ 109,00

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About This Item

CAS Number:

604-44-4

NACRES:

NA.52

PubChem Substance ID:

24893047

UNSPSC Code:

12352200

MDL number:

MFCD00003974

Key Documents

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grade

Molecular Biology

Quality Level

200

form

liquid

storage temp.

2-8°C

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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1 of 4

This Item	N7660	185507	N2125
Sigma-Aldrich C8302 4-Chloro-1-naphthol solution	Sigma-Aldrich N7660 4-Nitrophenol solution	185507 2-Naphthol	Sigma-Aldrich N2125 Naphthol AS-BI phosphate
grade for molecular biology	grade -	grade -	grade -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form liquid	form liquid	form powder or flakes	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -	storage temp. −20°C

General description

4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.

Application

Suitable for visualizing protein-peroxidase conjugates in western blotting.

Biochem/physiol Actions

Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.

Other Notes

0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H312 + H332,H370

pcodes

P260 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P311

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

163.9 °F - closed cup

flash_point_c

73.3 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

67-56-1

CAS No.

rmu

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Enhanced monoterpene emission in transgenic orange mint (Mentha × piperita f. citrata) overexpressing a tobacco lipid transfer protein (NtLTP1).

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Planta, 252(3), 44-44 (2020-09-03)

Overexpression of the tobacco lipid transfer protein (NtLTP1) gene in transgenic orange mint resulted in enhanced accumulation of monoterpenes in the cavity of head cells of glandular trichomes, which resulted in enhanced emission of monoterpenes from transgenic orange mints. Plants

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Cell biology international, 44(5), 1142-1155 (2020-01-23)

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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

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