B4062

BRAF (416-766), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): BRAF1, MGC126806, MGC138284, RAFB1
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 63-85 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 63-85 nmol/min·mg
• mol wt: ~63 kDa
• UniProt accession no.: P15056
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... BRAF(673)

Description

Biochem/physiol Actions

BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals. Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)