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06699

Atto 532

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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1 mg
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€ 314,00
€ 157,00

About This Item

UNSPSC Code:
12352108
NACRES:
NA.25
MDL number:
MFCD05865400

€ 157,00

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product line

BioReagent

Quality Segment

100

assay

≥90% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 532 is a new label with high molecular absorption (115,000) and quantum yield (0.90) as well as sufficient Stoke′s shift between excitation and emission maximum. It is optimized for excitation with frequency doubled Nd:YAG-Laser, and is characterized by high photostability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 532 has been conjugated with the secondary antibodies for STED (stimulated emission depletion) microscopy and immunofluorescence studies.[1]

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Sigma-Aldrich

Sigma-Aldrich

06699

Atto 532

Sigma-Aldrich

Sigma-Aldrich

41051

Atto 488

Sigma-Aldrich

Sigma-Aldrich

88793

Atto 532 NHS ester

Sigma-Aldrich

Sigma-Aldrich

40692

Atto 532 DOPE

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

assay

≥90% (HPCE)

assay

≥90% (HPLC)

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥80.0% (HPCE)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN7135
BCCL2775
BCCK7929
BCCK3317
BCCK0534

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Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
Kiyoto Kamagata et al.
Journal of the American Chemical Society, 134(28), 11525-11532 (2012-06-14)
A method was developed to detect fluorescence intensity signals from single molecules diffusing freely in a capillary cell. A unique optical system based on a spherical mirror was designed to enable quantitative detection of the fluorescence intensity. Furthermore, "flow-and-stop" control
View All Related Papers

Global Trade Item Number

SKUGTIN
142026-25G04061833320204
142026-100G04061833544518
06699-1MG-F04061826749623

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