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PC548

Anti-Porin (Ab-5) (185-197) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-VDAC (Voltage-Dependent Anion Channel)

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Pack SizeSKUAvailabilityPrice
50 μg
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€ 394,00

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat
Application:
Citations:
19

€ 394,00


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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (2 µg/mL))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VDAC1(7416)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~31 kDa (VDAC) porin protein.
Recognizes the ~31 kDa porin protein.
This Anti-Porin (Ab-5) (185-197) Rabbit pAb is validated for use in Immunoblotting for the detection of Porin (Ab-5) (185-197).

Immunogen

Human
a synthetic peptide corresponding to amino acids 185-197 of human porin

Application


Immunoblotting (2 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Rat heart tissue

Other Notes

Crompton, M., et al. 1999. Biochem. J.341, 233.
Yu, W.H., et al. 1995. J. Biol. Chem.270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem.268, 23289.
The immunogen peptide is 100% conserved in all three isoforms of VDAC (VDAC1, VDAC2, and VDAC3). Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
324870PC138423500
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

-

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, rat

species reactivity

human, rat

species reactivity

human

species reactivity

mouse


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Takahiro Ishiwata et al.
PloS one, 7(8), e42118-e42118 (2012-08-08)
CryAB (HspB5) and HspB2, two small heat shock genes located adjacently in the vertebrate genome, are hypothesized to play distinct roles. Mice lacking both cryab and hspb2 (DKO) are viable and exhibit adult-onset degeneration of skeletal muscle but confounding results
Theresa Schöttl et al.
Molecular metabolism, 4(9), 631-642 (2015-09-29)
Several human and rodent obesity studies speculate on a causal link between altered white adipocyte mitochondria in the obese state and changes in glucose homeostasis. We here aimed to dissect whether alterations in white adipocyte mitochondrial respiratory function are a
Xiaoxiao Peng et al.
Scientific reports, 6, 28080-28080 (2016-06-28)
Recently thioredoxin reductase 1 (TrxR1), encoded by Txnrd1, was suggested to modulate glucose and lipid metabolism in mice. Here we discovered that TrxR1 suppresses insulin responsiveness, anabolic metabolism and adipocyte differentiation. Immortalized mouse embryonic fibroblasts (MEFs) lacking Txnrd1 (Txnrd1(-/-)) displayed



Global Trade Item Number

SKUGTIN
PC548-50UG04055977207781

Questions

  1. I have used this antibody but I not only get a band at ~35kDa. I get a stronger band at ~100kDa. I just wanted to know if this is something that is known to happen with this antibody and what this off-target band might be. Many thanks for any help.

    1 answer
    1. The product issue you are reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

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