1.02482

Cellvento^® CHO-110

Chemically defined cell culture medium, dry powder

• UNSPSC Code: 41106213

Properties

• Quality Segment: 500
• description: shipped with cooling packs
• product line: Cellvento^®
• form: dry powder
• technique(s): cell culture | mammalian: suitable (suspension)
• application(s): pharma/biopharma processes
• storage temp.: 2-8°C

Description

General description

Cellvento^® CHO-110 chemically defined cell culture medium has been specially developed for the growth of Chinese Hamster Ovary (CHO) cells and the expression of monoclonal antibodies and recombinant proteins in suspension culture. The formulation is of non-animal origin, chemically defined and contains no hydrolysates or components of unknown composition. Cellvento^® CHO-110 medium has been formulated without L-glutamine, hypoxanthine, and tymidine to keep flexibility in applications. It is available in dry powder form or as ready-to-use medium to fit to different experimental set-ups.

Application

Cellvento^® CHO-110 medium:

• has been designed to support optimized cell growth and performance with DHFR-negative CHO suspension cell types, primarily recombinant CHODG44 cell lines, but may be suitable for use with other CHO cell lines.
• should be used for cell adaptation and cell bank generation.
• is suitable for production in batch and perfusion culture applications.
• is suitable for seed train expansion up to the N-2 step for fed-batch processes, switching to Cellvento^® CHO210 medium at the final N-1 expansion step, which is then used in conjunction with Cellvento^® Feed-210 companion feed in the final production culture.

This product is intended for research or further manufacturing but not for human or therapeutic use.

Preparation Note

Formulated to contain 27.5 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• pcodes: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• hcodes: H317,H319