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HPLC Analysis of Plasmids on TSKgel® DNA-NPR

HPLC Analysis of Plasmids on TSKgel® DNA-NPR application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

818249

TSKgel® DNA-NPR HPLC Column

phase DEAE (diethylaminoethyl), L × I.D. 7.5 cm × 4.6 mm, 2.5 μm particle size

Komponente der mobilen Phase

Produkt-Nr.
Beschreibung
Preisangaben

S3014

Natriumchlorid

Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

10812846001

Tris-Hydrochlorid

>99% (titration), pH 7.0-9.0, suitable for FISH

Vorsäule

Produkt-Nr.
Beschreibung
Preisangaben

818253

TSKgel® DEAE-NPR-Schutz-Säule

phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 2.5 μm particle size

CONDITIONS

column

TSKgel DNA-NPR, 7.5 cm x 4.6 mm, 2.5 μm particles (818249)

mobile phase

[A] 0.02 M Tris, pH 9.0; [B] 0.02 M Tris, 1.0 M NaCl, pH 9.0

gradient

50 to 60% B in 10 column volumes

flow rate

1 mL/min

detector

UV, 260 nm

sample

PUC19 plasmid

Beschreibung

Sonstige Hinweise

Data provided by Tosoh Bioscience LLC.

Rechtliche Hinweise

TSKgel is a registered trademark of Tosoh Corporation

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Related Applications

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