HPLC Analysis of Penicillamine Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 0.1% triethylamine acetate, pH 3.8; [B] ethanol; (20:80, A:B)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 225 nm

injection

5 μL

sample

5 mg/mL in methanol