HPLC Analysis of Oxprenolol Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

(A) acetonitrile; (B) methanol; (C) acetic acid; (D) triethylamine; (54.5:45:0.3:0.2, A:B:C:D)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

1 mg/mL in methanol