HPLC Analysis of Isoleucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C )
Materialien
Analytische Säule
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Standard
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L-Isoleucin
from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%L-Isoleucin
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandVorsäulenkartusche
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Astec® Chirobiotic® T Chiral HPLC Guard Column
Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mmCONDITIONS
column
Astec CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 205 nm
injection
10 μL
sample
300 μg /mL in 30:70 water:methanol
Beschreibung
Rechtliche Hinweise
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC