HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (23 °C)
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] 160 mM sodium phosphate, pH 4.5; [B] ethanol; (40:60, A:B)
flow rate
1 mL/min
column temp.
23 °C
detector
UV, 205 nm
injection
5 μL
sample
1 mg/mL in methanol
Beschreibung
Rechtliche Hinweise
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC