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  • First molecular identification of Entamoeba polecki in a piglet in Japan and implications for aggravation of ileitis by coinfection with Lawsonia intracellularis.

First molecular identification of Entamoeba polecki in a piglet in Japan and implications for aggravation of ileitis by coinfection with Lawsonia intracellularis.

Parasitology research (2015-05-13)
Makoto Matsubayashi, Kenta Kanamori, Masayuki Sadahiro, Masaharu Tokoro, Niichiro Abe, Makoto Haritani, Tomoyuki Shibahara
ZUSAMMENFASSUNG

Parasitic Entamoeba spp. are found in many vertebrate species including humans, as well as many livestock including pigs. In pigs, three Entamoeba spp., E. suis, and E. polecki and E. histolytica as zoonotic species, have been identified, but their pathogenicity has not been fully characterized. Here, we report the bacteriological, virological, and histopathological examination of three piglets with chronic diarrhea. Two animals appeared to be additionally infected with Lawsonia intracellularis, which caused a characteristic proliferative ileitis. In the piglet infected with Entamoeba spp., the trophozoites (approximately 10-15 μm with one nucleus in their cytoplasm) invaded into the lamina propria and the disease was worsened by the formation of ulcers and pseudomembranes. Genetic analysis identified the parasite as E. polecki (99.5% identity). Although E. polecki in humans or animals might be less pathogenic in the case of a single infection, coinfections with other pathogens including L. intracellularis may increase the severity of the disease.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Formaldehyd -Lösung, for molecular biology, 36.5-38% in H2O
Sigma-Aldrich
Ethidiumbromid -Lösung, BioReagent, for molecular biology, 10 mg/mL in H2O
Sigma-Aldrich
Formaldehyd -Lösung, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Sigma-Aldrich
Ethidiumbromid -Lösung, BioReagent, for molecular biology, 500 μg/mL in H2O
Sigma-Aldrich
Ethidiumbromid, BioReagent, for molecular biology, powder
Sigma-Aldrich
Formaldehyd -Lösung, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sigma-Aldrich
Ethidiumbromid -Lösung, for fluorescence, ~1% in H2O
Sigma-Aldrich
Formaldehyd -Lösung, meets analytical specification of USP, ≥34.5 wt. %
Sigma-Aldrich
Ethidiumbromid, ~95% (HPLC)
Sigma-Aldrich
Formaldehyd-12C -Lösung, 20% in H2O, 99.9 atom % 12C
Supelco
Pyridaben, PESTANAL®, analytical standard