Direkt zum Inhalt
Merck

RNA SHAPE analysis in living cells.

Nature chemical biology (2012-11-28)
Robert C Spitale, Pete Crisalli, Ryan A Flynn, Eduardo A Torre, Eric T Kool, Howard Y Chang
ZUSAMMENFASSUNG

RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
SHAPE In-vivo-Reagens für die RNA-Strukturanalyse in Lebendzellen, permits the analysis of RNA structure in living cells
Sigma-Aldrich
2-Methylnicotinic acid imidazolide