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V900483

Trizma® Base

≥99%, crystalline powder, reagent grade, Vetec™

Synonym(e):

2-Amino-2-(hydroxymethyl)-1,3-propandiol, THAM, Tris Base, Tris(hydroxymethyl)aminomethan, Trometamol

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Über diesen Artikel

Lineare Formel:
NH2C(CH2OH)3
CAS-Nummer:
Molekulargewicht:
121.14
UNSPSC Code:
12161700
PubChem Substance ID:
EC Number:
201-064-4
Beilstein/REAXYS Number:
741883
MDL number:
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Produktname

Trizma® Base, Vetec™, reagent grade, ≥99%

grade

reagent grade

description

aminopeptidase substrate

product line

Vetec™

assay

≥99%

form

crystalline powder

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

General description

Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma® base can be used as a buffer:
  • In the evanescent absorption analysis of NADPH.
  • In the autoxidation studies of oxymyoglobin.

Other Notes

Die pH-Werte aller Puffer sind temperatur- und konzentrationsabhängig. Bei Tris-Puffern steigt der pH-Wert um etwa 0,03 Einheiten pro °C Temperaturabfall und fällt 0,03-0,05 Einheiten pro Zehnfachverdünnung.
Verwenden Sie für präzise Anwendungen ein sorgfältig kalibriertes pH-Messgerät mit einer Glas/Kalomel-Kombielektrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany


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Lagerklasse

11 - Combustible Solids

Was ist los?

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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