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V900483

Sigma-Aldrich

Trizma® Base

≥99%, crystalline powder, reagent grade, Vetec™

Synonym(e):

2-Amino-2-(hydroxymethyl)-1,3-propandiol, THAM, Tris Base, Tris(hydroxymethyl)aminomethan, Trometamol

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About This Item

Lineare Formel:
NH2C(CH2OH)3
CAS-Nummer:
77-86-1
Molekulargewicht:
121.14
Beilstein:
741883
EG-Nummer:
201-064-4
MDL-Nummer:
MFCD00004679
UNSPSC-Code:
12161700
PubChem Substanz-ID:
329829884
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

Trizma® Base, Vetec™, reagent grade, ≥99%

Qualität

reagent grade

Beschreibung

aminopeptidase substrate

Produktlinie

Vetec™

Assay

≥99%

Form

crystalline powder

pH-Wert

10.5-12

Nützlicher pH-Bereich

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp (Schmelzpunkt)

167-172 °C (lit.)

Löslichkeit

water: 0.667 g/mL, clear, colorless to faintly yellow

SMILES String

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChIKey

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Allgemeine Beschreibung

Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.[1][2]

Anwendung

Trizma® base can be used as a buffer:
  • In the evanescent absorption analysis of NADPH.[3]
  • In the autoxidation studies of oxymyoglobin.[4]

Sonstige Hinweise

Die pH-Werte aller Puffer sind temperatur- und konzentrationsabhängig. Bei Tris-Puffern steigt der pH-Wert um etwa 0,03 Einheiten pro °C Temperaturabfall und fällt 0,03-0,05 Einheiten pro Zehnfachverdünnung.
Verwenden Sie für präzise Anwendungen ein sorgfältig kalibriertes pH-Messgerät mit einer Glas/Kalomel-Kombielektrode.

Rechtliche Hinweise

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
WXBF5258V
WXBF2513V
WXBF4131V
WXBF2803V
WXBF2802V

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Die Dokumentenbibliothek aufrufen

Tapered fibers for cell studies
Reviews in Fluorescence (2005)
Preparation of buffers for use in enzyme studies
Handbook of Biochemistry and Molecular Biology (2010)
Autoxidation of native oxymyoglobin. Kinetic analysis of the pH profile
Shikama K and Sugawara Y
European Journal of Biochemistry, 91(2), 407-413 (1978)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Corey A Baron et al.
Magnetic resonance in medicine, 73(3), 1075-1084 (2014-04-12)
An acquisition method that does not increase scan time or specific absorption rate is investigated for reducing the deleterious effects of cerebrospinal fluid (CSF) partial volume effects on diffusion tensor imaging (DTI) tractography. It is based on using a shorter
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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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