807477

TOYOPEARL^® Hydrophobe Wechselwirkung (HIC) Medien

matrix polymer (65 μm), bottle of 100 mL, 65 μm

• Synonym(e): TOYOPEARL^® Butyl-650M Bulk-Medien
• UNSPSC Code: 41115700
• NACRES: NB.21
• Particle size: 65 μm
• Matrix active group: C4 phase
• Matrix: polymer (65 μm)

Eigenschaften

• description: Butyl-650M
• product line: TOYOPEARL^®
• form: slurry
• mol wt: 2000 - 1,000,000
• packaging: bottle of 100 mL
• parameter: 3 bar max. pressure
• technique(s): HPLC: suitable
• matrix: polymer (65 μm)
• matrix active group: C4 phase
• particle size: 65 μm
• operating pH: 1-13
• capacity: 40 g/L±10 adsorption capacity (lysozyme)
• separation technique: hydrophobic interaction (HIC)

Beschreibung

General description

Die Toyopearlpackungen für die hydrophobe Wechselwirkungschromatographie (HIC) bieten folgende Vorteile:

• Starke Affinität zu wasserlöslichen Proteinen
• Hohe Wiederfindung von Masse und Aktivität
• Hohe Probenkapazität - das 2-4-fache gebräuchlicher Gele
• Schwankende Salzkonzentrationen verändern das Bettvolumen nicht
• Mechanisch stabil bis 7 kg/cm² (7 bar, 100 psi)
• pH-Bereich 2-12
• Mit 0,05 M NaOH auf der Stelle gereinigt
• Autoklavierbar

Toyopearl-Medien bieten eine hohe Wiederfindung von Proteinen, wobei verschiedene wässrige Eluenten verwendet werden. Die Harze haben eine Ausschlußgrenze von 5 × 10⁶ Dalton. Die große Porenweite von 1000 Å ermöglicht es diesen Materialien, sehr große Proteine mittels eines hydrophoben Wechselwirkungsmechanismus zu trennen, ohne daß Größenausschlußeffekte eintreten.
100 mL Flasche.

TOYOPEARL^® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.

Application

TOYOPEARL^® media are used in hydrophobic interaction media, resins and separation media. TOYOPEARL^® media offer high yield recovery of proteins, using various aqueous eluants.

Features and Benefits

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• Gefahrencodes: H226
• Sicherheitshinweise: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Was ist los?: WGK 1
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)