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Merck

807469

TOYOPEARL® HW-75F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 45 μm particle size

Synonym(e):

TOYOPEARL® HW-75F Bulk-Medien

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About This Item

UNSPSC-Code:
41115700

Beschreibung

HW-75F

Produktlinie

TOYOPEARL®

Form

slurry

Verpackung

bottle of 500 mL

Parameter

3 bar max. pressure

Methode(n)

HPLC: suitable

Matrix

hydroxylated methacrylic polymer

Aktive Matrixgruppe

hydroxylated methacrylate phase

Partikelgröße

45 μm

Porengröße

100,000-10,000,000 Da MW range

Betriebs-pH-Wert

2-13

Trenntechnik

size exclusion (SEC)

Allgemeine Beschreibung

Toyopearl HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.

Anwendung

TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Spezifikationen

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physikalische Form

Shipped in 20% (v/v) ethanol.

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Rechtliche Hinweise

Toyopearl is a registered trademark of Tosoh Corporation

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Flam. Liq. 3

WGK

WGK 1

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin

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