796-0659

HPLC Analysis of Human Growth Hormone (hGH) on Discovery^® BIO PolyMA-WAX

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm I.D., 5 μm particles (59602-U); mobile phase: [A] 20 mM Tris-HCl, pH 8.0; [B] 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0; gradient: 5 to 70% B in 30 min. (linear); flow rate: 0.5 mL/min; column temp.: 25 °C; detector: UV, 210 nm; injection: 10 mg hGH
• Eignung: application for HPLC
• Anwendung(en): life science and biopharma

Beschreibung

Hinweis zur Analyse

This Figure shows the degradation products of human growth hormone (hGH) well-separated on a Discovery BIO PolyMA-WAX anion-exchange column. The difference between the resolved compounds is the conversion of protein amide(s) to carboxylate(s), demonstrating both the power of the ion-exchange technique, and the efficiency of the Discovery BIO PolyMA-WAX column.

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

Human Growth Hormone human, HGH, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture