ZRB2535

Anti-IL-10 Antibody, clone 1O10 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Interleukin-10;Cytokine synthesis inhibitory factor;CSIF
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1O10, recombinant monoclonal
• Application: FACS, ICC, IHC (p), WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1O10, recombinant monoclonal
• description: 1O10 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 20.52 kDa, observed mol wt ~15 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_000563
• UniProt accession no.: P22301
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... IL10(3586)

Beschreibung

General description

Interleukin-10 (UniProt: P22301; also known as IL-10, Cytokine synthesis inhibitory factor, CSIF) is encoded by the IL10 gene (Gene ID: 3586) in human. IL-10 is a homodimeric secreted anti-inflammatory cytokine produced by a variety of cells, including T-cells, macrophages, and mast cells.  It is a major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions.  It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form. IL-10 acts on many cells of the immune system to limit excessive tissue disruption caused by inflammation. During infection, it inhibits the activity of Th1 cells, NK cells, and macrophages, all of which are required for optimal pathogen clearance. It inhibits the release of pro-inflammatory cytokines.  It ameliorates the excessive Th1 and CD8+ T cell responses that are responsible for much of the immunopathology associated with infections. It is shown to control the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. IL-10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB, which leads to JAK1 and STAT2-mediated phosphorylation of STAT3 that in turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Couper, KN., et al. (2008). J. Immunol. 180(9); 5771-5777; Yoon, SI., et al. (2006). J. Biol. Chem. 281(46); 35088-35096).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

Full-length mature (without the signal peptide) recombinant human IL-10.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human IL-10.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected full-length, mature, recombinant Human IL-10 (without the signal peptide).

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human IL-10 with a KD of 1.0 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution from a representative lot detected IL-10 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution o from a representative lot detected IL-10 in Jurkat cells.

Flow Cytometry Analysis: 1 µg from a representative lot detected IL-10 in one million Human peripheral blood mononuclear cells (PBMC) treated with phorbol 12-myristate 13-acetate( PMA; 500 ng/mL), Ionomycin (500 ng/mL) and Brefeldin A (100 ng/mL) for 4- 6 hours.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 1O10 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects IL-10.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable