ZRB1932

Anti-TBKBP1 Antibody, clone 3F13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): TANK-binding kinase 1-binding protein 1;TBK1-binding protein 1
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 3F13, recombinant monoclonal
• Application: IHC, WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3F13, recombinant monoclonal
• description: 3F13 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 67.7 kDa, observed mol wt ~75 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): mouse, porcine, canine, rat, bovine, feline
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_055541
• UniProt accession no.: A7MCY6
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TBKBP1(9755)

Beschreibung

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

TANK-binding kinase 1-binding protein 1 (UniProt: A7MCY6; also known as TBK1-binding protein 1) is encoded by the TBKBP1 (also known as KIAA0775, SINTBAD) gene (Gene ID: 9755) in human. TBKBP1 is an adapter protein that constitutively binds TANK-binding kinase 1 (TBK1) and Inhibitor of NF-kB kinase subunit epsilon (IKBKE) and plays a role in antiviral innate immunity. Its TBK1 and IKBKE interacting region is localized in amino acids 280-329. It can exist as a homodimer or can form heterodimer with NAP1. Its homodimerization region is localized in amino acids 1-279. TBKBP1 expression is observed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, muscle, heart, and brain. TBK1 that is reported to be a central mediator of growth factor signaling relies on TBKBP1 for its action. TBKBP1 recruits TBK1 to protein kinase C (PKC ) through a scaffold protein, caspase recruitment domain 10 (CARD10). This enables PKC to phosphorylate TBK1 at serine 716. This phosphorylation is essential for TBK1 activation by growth factors, but not by innate immune stimuli. It has been reported that although the TBK1-TBKBP1 signaling axis is not essential for the induction of type I interferon, it facilitates mTORC1 activation and oncogenesis. Deletion of either TBK1 or TBKBP1 is known to inhibit tumorigenesis in a murine model of lung cancer. The TBK1-TBKBP1 axis can also facilitate tumor-mediated immunosuppression via induction of PD-L1 and stimulation of glycolysis. Two isoforms of TBKBP1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhu, L., et al. (2019). Nat. Cell Biol. 21; 1604-1614; Goncalves, A., et al. (2011). PLoS One. 6(9); e23971).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human TANK-binding kinase 1-binding protein 1 (TBKBP1).

Application

Quality Control Testing

Evaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TBKBP1 in NTERA-2 cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound TBKBP1 peptide with a KD of 2.1 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TBKBP1 in human placenta tissue sections.

Western Blot Analysis: A 1:1000 dilution of a representative lot of the this antibody detected TBKBP1 in HEK293T cells transfected with a TBKBP1 expression vector.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Clone 3F13 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects TANK-binding kinase 1-binding protein 1 (TBKBP1). It targets an epitope within 17 amino acids from the C-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable